Cell line development is a crucial step in the biopharmaceutical industry. Achieving stable and efficient expression of target proteins is one of the key factors for successful cell line development. Site-specific integration technology is an important method in cell line development, and its importance is reflected in the following aspects:
First, it improves gene insertion stability. Random integration technology is a classic method in cell line development, but its insertion site is unstable, resulting in common problems such as fluctuation and loss of gene expression. Using site-specific integration technology can accurately integrate exogenous genes into specific positions on the target cell chromosome, ensuring the stability of gene expression and greatly reducing the uncertainty in the process of cell line development.
Second, it reduces gene toxicity reactions. Random integration technology may cause exogenous genes to be inserted into the promoter or transcription factor region, leading to toxic reactions. Site-specific integration technology can avoid this problem, not only ensuring the stability of gene expression, but also reducing the risk of gene toxicity reactions.
Third, it improves gene expression efficiency. Using site-specific integration technology, high-expression cell lines of target proteins can be quickly obtained by screening clones integrated into the target site, thereby improving gene expression efficiency. Efficient gene expression is critical to produce high-quality biologics, especially for biopharmaceuticals that require large-scale production.
Fourth, it reduces production costs. Site-specific integration technology can precisely regulate the expression level of the target gene, thereby better controlling the production process and product quality. This reduces unnecessary waste and repetitive work, and also increases production efficiency, reducing production costs.
Fifth, it improves product quality. Site-specific integration technology can precisely control the expression level and quality of the target gene, playing a key role in producing high-quality biologics. For example, targeting the instability and cleavage of single elements, reducing impurity levels, and ensuring the stability and consistency of product quality. It also provides more options for biopharmaceutical companies, enabling them to better meet market and customer needs.
In summary, using site-specific integration technology in cell line development has significant advantages, including improving gene insertion stability, reducing gene toxicity reactions, improving gene expression efficiency, reducing production costs, and improving product quality. These advantages make site-specific integration technology an irreplaceable important technology in the biopharmaceutical industry.
Post time: Jun-01-2023